ABSTRACT
This part is central for the book. It contains detailed descriptions and outlines of more than 900 enzyme-specific zymogram techniques suitable for visualization on electrophoretic gels of more than 400 different enzymes. The information presented herein is traditionally arranged around the “enzyme sheet,” which includes basic information required for the detection of a particular enzyme. At the beginning of this part, additional information of general relevance to the enzyme sheets is given. It includes a description of the structure of an enzyme sheet, general considerations, and comments and recommendations concerning application of zymogram techniques, as well as resource-saving strategies, troubleshooting, and safety measures. Abbreviations commonly used in the enzyme sheets are also given.
