ABSTRACT
The objective of this part is to describe the general principles involved in visualization of gel areas containing specific enzymes separated during gel electrophoresis. These principles may be classified as resulting in positive or negative zymograms, as chemical or physical, as chromogenic or fluorogenic, as based on chemical coupling or enzymatic coupling, etc. It seems, however, that the mixed operational classification will be preferable for purely practical consideration. According to this classification, the main principles of enzyme visualization on electrophoretic gels are defined as:
Based on chromogenic reactions
Based on fluorogenic reactions
Autoradiography
Bioautography
Two-dimensional gel spectroscopy
Immunoblotting
Miscellanies
