Soil samples collected from the different sources are serially diluted in sterile phosphate buffered saline (PBS) up to 10−7. From the dilution, 100 pl is plated on Luria Bertani (1951) agar (LBA) plates and incubated overnight at 37°C. Independent pinhead bacterial colonies are collected and liquid cultured in 10 ml sterile tubes containing LB broth. All the pure colonies are screened for lipase production on tributyrin agar (TBA) 142plates at 37°C for 72 h on the basis of formation of zone of hydrolysis surrounding the bacterial colony.