Research work was conducted in six rice growing districts of Punjab, covering three flood plains (Ravi, Chenab and Jhelum) to investigate the geographic variations of arsenic (As) in irrigation well water. In field, irrigation well water was tested using ITS Field Kit which was later analyzed by ICP-MS at Columbia University, New York, USA. Results indicate that Ravi flood plain is different from Chenab and Jhelum Flood plain; with Ravi floodplain had a higher As concentration in irrigation wells than those from the Chenab and Jhelum Flood plain and high As wells was concentrated near the river bank. The results of this study form a strong basis for the researchers in designing detailed studies on the accumulation of As in paddy soil and possible uptake by rice.