Arsenic (As) accumulation in rice is a severe problem in Southeast Asia including West Bengal, India. Thiourea (TU) is a redox active thiol (-SH) based molecule, which has been found to regulate redox equilibrium and As level in lab study. In this work, we, for the first time, evaluated the effect of TU supplementation on growth and yield of rice plants, biochemical changes and As concentrations in rice grains. Both rice cultivars (Satabdi-IET4786 and Gosai) were grown in three fields (Control, Low As and High As) in Nadia, West Bengal. The result indicated positive impact of TU supplementation on rice growth and yield and most importantly, showed reduction in As concentrations in rice grains.