Environmental pollution of arsenic (As) is a serious problem that is not only affecting the local people but also population throughout the world through rice and rice-based food products. Rice accumulates As in significantly greater amounts in comparison to other cereal crops. Microbiological interventions can serve as protective agents to ameliorate As stress in rice. The experiments were conducted in hydroponically grown in rice seedlings subjected to arsenate [As(V)] stress for 15 d. Two-dimensional gel electrophoresis analysis of 340 spots revealed that 145 spots were differentially expressed; out of these, 87 spots were identified using MALDI-TOF-TOF. The differentially expressed proteins during supplementation of microbial consortium under As stress belonged to defense, growth promotion, energy metabolism, cellular and metabolic processes.