Arsenic (As) is a toxic element, which poses significant threat to human health due to frequency of occurrence, toxicity and various routes for human exposure. Arsenic contamination of water and food, especially rice, is a serious issue. Selenium (Se) is an essential element for human and other animals and is beneficial for plants as well. Selenium has been found to antagonize the toxicity of As in plants. The proteomics study was performed by using 2-dimensional gel electrophoresis and MALDI-TOF/TOF. The present study provides new insights into As-Se interaction in rice leaves at the proteome level and identifies crucial proteins involved in Se mediated amelioration of As toxicity in rice.