In this study we investigated accumulation of selenium and zinc by cells and certain organic compounds, pigments content, activity of energy metabolism enzymes and antioxidant system in Chlorella vulgaris Beij. We observed samples after adding sodium selenite in concentration of10.0 mg/dm3 and Zn2+ - 5.0 mg/dm3 during the next 7 days. It was discovered that content of selenium in Ch. vulgaris cells increased more significantly after adding sodium selenite in combination with zinc, than in case when we added only selenite, and that the accumulation of Zn2+ in the algae cells was very intensive. Lipids in comparison to proteins accumulate selenium in 58% more after adding sodium selenite alone, and in 10% more after simultaneous action of sodium selenite and zinc.
The content of pigments, after adding sodium selenite alone and combination of sodium selenite with zinc ions, increased in comparison to the control, chlorophyll a by 23.1% and 41.2%, chlorophyll b in 1.3 and 2.5 times, carotenoids by 46.6%, and 70.6%, respectively. Content of pheopigments decreased by 7.7% after adding selenite and by 28.4% in a result of the combined effect of Se (IV) and Zn (II) in comparison to the control sample.
Sodium selenite when added alone as well as in combination with zinc, stimulated energy metabolism of chlorella by activating cytochrome oxidase and succinate dehydrogenase, NADH- and NADPH-glutamate dehydrogenases as well as the enzyme activity of antioxidant system.