It is established that purple coneflower (Echinacea purpurea (L.) Moench) and pale purple coneflower (Echinacea pallida [Nutt.] Nutt.) contains specific protein compound—lectins, which have the ability to reversibly interact with carbohydrates and exhibit high biological activity at the cellular and organism levels. An original methodology of hemagglutination activity of Echinacea extracts evaluation was developed. The study of the dynamics of lectin activity showed that in the E. purpurea high level of hemagglutinins was found in roots with rhizomes, stems, both forming an open inflorescences. It has been established that during the vegetation of E. pallida the highest level of phytolectins observed in leaves—twice as much higher as in roots with rhizomes. In the genesis period, hemagglutination activity of herb extracts was higher than roots with rhizomes extracts. Echinacea overground mass considered as important raw materials for preparative isolation of lectins.439