To assess the fate of residually added selenium (Se) fertilizer in soil over time, many studies have used chemical extraction methods to quantify “available” Se (Dhillon et al. 2005, Keskinen et al. 2009). Few studies have used plant evaluation trials to assess the bioavailability of the added Se in different soils (Supriatin et al. 2015), and even fewer have assessed changes in bioavailability of Se in soil over time (Li et al. 2016). Our experiment aimed to assess how Se transfers from soil to plant over a total period of 12 months. By using both chemical extraction methods and a pot experiment, we investigated (1) the chemical extraction method which gave the most consistent prediction for plant Se uptake and (2) the effect of soil properties on time-dependent Se fixation. Results from this study can be used in conjunction with others to provide a more comprehensive understanding of residual fate and bioavailability of Se.