Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

chapter 2|16 pages

Characteristics of cells in culture

chapter 4|23 pages

Growth and maintenance of cells in culture

chapter 5|33 pages

Cell line and culture monitoring

chapter 8|24 pages

Hybridomas—sources of antibodies

chapter 9|26 pages

Scaling up animal cell culture

chapter 10|25 pages

Modes of culture for high cell densi

chapter 11|11 pages

Production from cell culture