High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the

Fundamentals. Monolithic Stationary Phases in HPLC. Bonded Stationary Phases. Micro-HPLC Chromatography. Two-Dimensional Comprehensive Liquid Chromatography. Gradient Elution Mode. Capillary Electromigration Techniques. LC-MS Interfaces: State of the Art and Emerging Techniques. Control and Effects of Temperature in Analytical HPLC. Nonlinear Liquid Chromatography. Displacement Chromatography in the Separation and Characterization of Proteins and Peptides. Field-Flow Fractionation. Affinity Chromatography. Ion Chromatography: Modes for Metal Ions Analysis. Retention Models for Ions in HPLC. Polymer HPLC. Applications. HPLC in Chiral Pharmaceutical.HPLC in Environmental Analysis. HPLC in Food Analysis. HPLC in Forensic Sciences.