First published in 1989, written with contributions from experts who have critically evaluated the state of the art and the prospects of isolated adults cardiac myocytes in cardiological research, this two-volume publication summarizes the current knowledge of this technology. The methodology, structure, and metabolism of cardiomyocytes are presented, along with metabolism and cation homeostasis and energy metabolism and hypoxic injury. Additionally, cell isolation and culture and the structure and biochemistry of the sarcolemma is explained. Electrophysiology and contractile function with ionic currents and membrane channels is discussed. Also, the electro-physiological effects of transmitters and hormones are presented, with excitation-contraction coupling and the use of cardiomyocytes in drug design and research explained.

part |80 pages

The Cardiomyocyte in Tissue and in the Isolated State

chapter Chapter 2|38 pages

Techniques for Isolation and Culture of Adult Cardiomyocytes

ByStuart L. Jacobson

part |42 pages

Cation Homeostasis

chapter Chapter 3|15 pages

Cation Homeostatis in Isolated Cardiac Myocytes

ByRuth A. Altschuld, Gerald P. Brierley

chapter Chapter 4|24 pages

Homeostasis of Calcium Ions in Calcium-Tolerant Myocytes

ByC. H. Fry, D. P. Harding

part |80 pages

Structure and Biochemistry of the Sarcolemma

chapter Chapter 5|19 pages

Ultrastructure of the Sarcolemma of Isolated Cardiomyocytes

ByJoy S. Frank

chapter Chapter 6|17 pages

Biochemistry of Sarcolemma of Isolated Cardiocytes

ByWilliam B. Weglicki, Jay H. Kramer, I. Tong Mak, Benjamin F. Dickens, Terence M. Phillips

chapter Chapter 7|40 pages

Adrenergic, Cholinergic, and Other Hormone Receptors on Cardiac Myocytes

ByDonald C. Bode, Laurence L. Brunton

part |54 pages

Energy Metabolism

chapter Chapter 8|10 pages

Oxygen Delivery and Oxidative Phosphorylation in Isolated Adult Ventricular Myocytes

ByBeatrice A. Wittenberg

chapter Chapter 9|24 pages

Metabolism of Purines by Adult Cardiomyocytes

ByJocelyn W. Dow

chapter Chapter 10|18 pages

Substrate Oxidation by Isolated Adult Cardiomyocytes

ByHans Michael Piper, Rolf Spahr

part |52 pages

Hypoxic Cell Injury

chapter Chapter 11|27 pages

Energy Metabolism and Hypoxic Injury of Cardiomyocytes

ByHans Michael Piper, Peter Schwartz, Berthold Siegmund

chapter Chapter 12|22 pages

Cytosolic Ca2+ in Hypoxic Cardiomyocytes

ByAshley P. Allshire, Peter H. Cobbold