ABSTRACT

This book will examine the relevant biological subjects involved in biomimetic microengineering as well as the design and implementation methods of such engineered microdevices. Physiological topics covered include regeneration of complex responses of our body on a cellular, tissue, organ, and inter-organ level. Technological concepts in cell and tissue engineering, stem cell biology, microbiology, biomechanics, materials science, micro- and nanotechnology, and synthetic biology are highlighted to increase understanding of the transdisciplinary methods used to create the more complex, robust biomimetic engineered models. The effectiveness of the new bioinspired microphysiological systems as replacements for existing in vitro or in vivo models is explained through sections that include the protocols to reconstitute three-dimensional (3D) structures, recapitulate physiological functions, and emulate the pathophysiology of human diseases. This book will also discuss how researchers can discover bridge technologies for disease modeling and personalized precision medicine.

Features

  • Focuses on cutting edge technologies that enable manipulation of living systems in a spatiotemporal manner.
  • Incorporates research on reverse engineering of comples microenvironmental factors in human diseases.
  • Highlights technologies related to patient-specific personalized medicine and their potential uses.
  • Written by chapter authors who are highly respected researchers in science and engineering.
  • Includes extensive references at the end of each chapter to enhance further study.

Hyun Jung Kim is an Assistant Professor in the Department of Biomedical Engineering at The University of Texas at Austin. After receiving hois Ph.D. degree at Yonsei University in the Republic of Korea, he did extensive postdctoral research at both the University of Chicago and the Wyss Institute at Harvard University. These efforts resulted in cutting-edge breakthroughs in synthetic microbial community research and organomimetic human Gut-on-a-Chip microsystem. His research on Gut-on-a-Chip technology leads to the creation of a microfluidic device that mimics the physiology and pathology of the living human intestine. Since 2015, he has explored novel human host-microbiome ecosystems to discover the disease mechanism and new therapeutics in inflammatory bowel disease and colorectal cancers at UT Austin. In collaboration with clinicians, his lab is currently developing disease-oriented, patient-specific models for the advancement in pharmaceutical and clinical fields.

part I|79 pages

Emulating the Microenvironment of a Living System

chapter 1|34 pages

Emulating Biomechanical Environments in Microengineered Systems

ByJason Lee, Lei Mei, Daniel Chavarria, Aaron B. Baker

chapter 2|24 pages

Biomimetic Microsystems for Blood and Lymphatic Vascular Research

ByDuc-Huy T. Nguyen, Esak Lee

chapter 3|19 pages

Multispecies Microbial Communities and Synthetic Microbial Ecosystems

ByJames Q. Boedicker

part II|132 pages

Enabling Technologies for Building a Biomimetic Model

chapter 4|29 pages

Stem Cell Engineering

ByYi Sun Choi, Kisuk Yang, Jin Kim, Seung-Woo Cho

chapter 5|28 pages

Organoid Technology for Basic Science and Biomedical Research

BySzu-Hsien (Sam) Wu, Jihoon Kim, Bon-Kyoung Koo

chapter 6|25 pages

Design, Fabrication, and Microflow Control Techniques for Organ-on-a-Chip Devices

ByZachary Estlack, Ali Khodayari Bavil, Jungkyu Kim

chapter 7|24 pages

Microfluidic Techniques for High-Throughput Cell Analysis

ByDongwei Chen, Juanli Yun, Yuxin Qiao, Jian Wang, Ran Hu, Beiyu Hu, Wenbin Du

chapter 8|22 pages

3D Printing and Bioprinting Technologies

BySe-Hwan Lee, Hyoryung Nam, Bosu Jeong, Jinah Jang

part III|73 pages

Pathomimetic Disease Modeling

chapter 9|22 pages

Microengineered Models of Human Gastrointestinal Diseases

ByWoojung Shin, Landon A. Hackley, Hyun Jung Kim

chapter 10|25 pages

Respiratory Pathophysiology

Microphysiological Models of Human Lung
ByBrian F. Niemeyer, Alexander J. Kaiser, Kambez H. Benam

chapter 11|23 pages

In Vitro Alzheimer’s Disease Modeling Using Stem Cells

ByHyun-Ji Park, Song Ih Ahn, Jeong-Kee Yoon, Hyunjung Lee, YongTae Kim

part IV|84 pages

Towards Translational Application and Precision Medicine

chapter 13|24 pages

Microarray 3D Bioprinting for Creating Miniature Human Tissue Replicas for Predictive Compound Screening

ByAlexander D. Roth, Stephen Hong, Moo-Yeal Lee

chapter 14|30 pages

Integration of the Immune System into Complex In Vitro Models for Preclinical Drug Development

ByJason Ekert, Sunish Mohanan, Julianna Deakyne, Philippa Pribul Allen, Nikki Marshall, Claire Jeong, Spiro Getsios